Tamoxifen-induced DNA adducts in endometrial samples from breast cancer patients.
نویسندگان
چکیده
Tamoxifen-induced DNA adducts were analyzed with the (32)P-postlabeling method using high-performance liquid chromatography (HPLC)-radioactivity detection from endometrial tissue of breast cancer patients and controls. Liver DNA from tamoxifen-treated rats was used as a positive standard. In blind analysis, five of the seven samples from tamoxifen-treated patients showed DNA adducts; none of the five controls were positive. The identity of the tamoxifen adduct was confirmed by using different chromatographic systems, isolating the HPLC fractions and running them on TLC, with or without spiked rat liver samples. The level of adducts in the treated patients was 2.7 adducts/10(9) nucleotides in the HPLC analysis.
منابع مشابه
Tamoxifen-induced DNA Adducts in Endometrial Samples from Breast Cancer Patients1
Tamoxifen-induced DNA adducts were analyzed with the 32P-postIabeling method using high-performance liquid chromatography (HPLQradioactivity detection from endometrial tissue of breast cancer patients and controls. Liver DNA from tamoxifen-treated rats was used as a positive standard. In blind analysis, five of the seven samples from tamox ifen-treated patients showed DNA adducts; none of the f...
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ورودعنوان ژورنال:
- Cancer research
دوره 56 19 شماره
صفحات -
تاریخ انتشار 1996